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(1) In case of weak Epi-Fluorescence
•Increase excitation light by removing ND filters ?Note that fading is accelerated in this case)
•If you use a mercury lamp for light source, reconfirm if the lamp is properly centered. It may be off from the best position due to deterioration of the lamp.
•Check if the suitable excitation filter is chosen. There is another choice of filter which has wider transmission range. ?ex? Change from EX450-490 to EX420-490?
•Check the barrier filter (emission filter) If you use a band pass type filter, try a filter with wider transmission range and see the result. Or the long pass type may do.
•Check what objective lenses are used. Choose an objective lens with a higher NA, the same magnification. Oil/Water immersion lenses are recommended for Epi-Fluorescent microscopy rather than dry objective lenses.
(2) In case of low contrast of Epi-Fluorescence.
•Check the barrier filter (emission filter). If you use a longpass type filter, try a bandpass type.
•An objective lens may be dirty. Also an image quality deteriorates markedly if some oil adheres to the tip of dry objective lens, or some water mix with the oil at the tip of oil-immersion objective. |
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